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17 Οκτ 2016 · In our search to identify and overcome key problematic steps when extracting DNA/RNA from inhibitor-rich soil samples, we found that commercially available nucleic acid extraction/purification kits are not always better than non-kit methods (e.g., Nicolaisen’s method).
Transparent DNA/RNA Co-extraction Workflow Protocol Suitable for Inhibitor-Rich Environmental Samples That Focuses on Complete DNA Removal for Transcriptomic Analyses. METHODS. published: 18 October 2016 doi: 10.3389/fmicb.2016.01588.
18 Οκτ 2016 · Abstract. Adequate comparisons of DNA and cDNA libraries from complex environments require methods for co-extraction of DNA and RNA due to the inherent heterogeneity of such samples, or risk bias caused by variations in lysis and extraction efficiencies.
Various techniques are used to extract different types of DNA (Figure \(\PageIndex{2}\)). Most nucleic acid extraction techniques involve steps to break open the cell, and then the use of enzymatic reactions to destroy all undesired macromolecules.
14 Νοε 2012 · This study describes a rapid and reliable cetyl trimethylammonium bromide (CTAB) protocol suited specifically for extracting DNA from plants which are rich in polysaccharides and secondary metabolites, and the protocol also excludes the use of expensive liquid nitrogen and toxic phenols.
18 Οκτ 2016 · Here, we tested several DNA/RNA co-extraction commercial kits on inhibitor-rich soils, and compared them to a commonly used phenol-chloroform co-extraction method.
There are five basic steps of DNA extraction that are consistent across all the possible DNA purification chemistries: 1) disruption of the cellular structure to create a lysate, 2) separation of the soluble DNA from cell debris and other insoluble material, 3) binding the DNA of interest to a purification matrix, 4) washing proteins and other ...
