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  1. 22 Ιουλ 2021 · Excel file format cannot be determined, you must specify an engine manually. When all files are closed and no hidden temporary files ~$filename.xlsx in the same directory the code works perfectly. Share

  2. To solve the "ValueError: Excel file format cannot be determined, you must specify an engine manually" error, make sure: To ignore all temporary files that start with ~$ , e.g. ~$file_name.xlsx . To use the correct pandas method when reading .xlsx and .csv files.

  3. 19 Ιουν 2023 · This error occurs when Pandas is unable to determine the file format of an Excel file and needs the engine to be specified manually. In this blog post, we will explore the cause of this error and provide a step-by-step guide on how to fix it.

  4. 23 Ιουλ 2023 · The error message “ValueError: Excel file format cannot be determined, one must specify an engine manually” occurs when attempting to read an Excel file in Python, but the library being used is unable to automatically determine the file format.

  5. 27 Σεπ 2022 · Today, a plethora of different bioinformatic and molecular file formats are used, from the simplest FASTA format, which includes sequence data with a description, to more complex formats such as General Feature Format (GFF), which displays detailed genomic features.

  6. 1 Ιαν 2020 · Each chapter explains statistical formulas and directs the reader to use Excel commands to solve specific, easy-to-understand biological and life science problems.

  7. Try the fixes here: https://stackoverflow.com/questions/68478097/pandas-glob-excel-file-format-cannot-be-determined-you-must-specify-an-engi. As is, since this bug report only has the subject line filled in, there's nothing to really go on in terms of troubleshooting or debugging.