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Bromophenol blue (3′,3″,5′,5″-tetrabromophenolsulfonphthalein, BPB), [2] albutest [3] is used as a pH indicator, an electrophoretic color marker, and a dye. It can be prepared by slowly adding excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid.
Bromophenol Blue | C19H10Br4O5S | CID 8272 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
Bromophenol Blue (BPB): sulfonated hydroxyquinone, acts as a pH indicator and tracking dye in gel electrophoresis. Widely used in DNA, RNA, and protein analysis.
Bromophenol Blue is an indicator used in chemistry that changes color from yellow to dark blue in the presence of free amino groups, allowing for noninvasive and continuous detection of these groups. AI generated definition based on: The Combinatorial Index, 1998
Bromophenol blue is a pH indicator, yellow below pH 4 and blue above that pH. Therefore, if the SDS sample buffer turns yellow, the solution is still slightly acidic due to remaining TCA. Just add a small amount of 1 M Tris pH 8.0, until the color returns to the usual blue (do not use NaOH because you could hydrolyze the protein!).
Bromophenol blue runs roughly at the same position as the samples. Use a DNA gel loading buffer containing xylene cyanol or do not add any dye to it. Run an aliquot containing bromophenol blue next to your samples. View chapter Explore book.
ChemSpider record containing structure, synonyms, properties, vendors and database links for Bromophenol Blue, 115-39-9, 204-086-2
Bromophenol blue, Tetrabromophenolsulfonephthalein, is an acid-base indicator whose useful range as an indicator lies between pH 3.0 and 4.6. It changes from yellow at pH 3.0 to purple at pH 4.6; this reaction is reversible.
Bromophenol Blue is a sulfonated hydroxyquinone, tracking dye in DNA and RNA electrophoresis and a vital stain to probe the blood-brain barrier. Shop now at Sigma Aldrich.
A bromophenol is an organic compound consisting of hydroxyl groups and bromine atoms bonded to a benzene ring. They may be viewed as hydroxyl derivatives of bromobenzene , or as brominated derivatives of phenol .