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Fractionation of Cells. Although biochemical analysis requires disruption of the anatomy of the cell, gentle fractionation techniques have been devised to separate the various cell components while preserving their individual functions.
FRACTIONATION. After homogenization, the cellular components need to be separated/fractionated. Again, the pro-tocols in this manual, for the most part, use the same technique: differential centrifugation. During centrifugation, cellular components are pelleted based on size, density, and sedimentation properties.
17 Αυγ 2007 · This chapter presents techniques for the fractionation of cellular components, including organelles and appendages, beginning with external surfaces and ending with internal components.
1 Φεβ 2011 · For providing evidence for the most reliable and efficient separation technique, we compared four different procedures for subcellular fractionation of Jurkat cells.
Four great things about subfractionation: Study single biological processes free from other interfering reactions in the cell w/o having to worry about keeping the cells alive-cell free system. Fractions are biologically active and can be stored. Minimal equipment needed and starting material is easily obtained.
This is a free sample of content from Subcellular Fractionation. Click here for more information on how to buy the book. © 2015 by Cold Spring Harbor Laboratory Press.
Cell fractionation is a process that allows scientists to separate different parts of a cell and study them individually. It involves breaking open cells, separating the components by centrifugation based on properties like density and size, and collecting the distinct fractions.
