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22 Μαρ 2020 · Resolving power refers to the ability of an optical instrument (e.g., the human eye, a microscope, or a telescope) to separate equal intensity point sources of light as two distinct points. Resolving power is a property of an optical instrument and the properties of light.
science and develops super-resolution fluorescence microscopy methods such as linear and nonlinear structured illumination, pointillism and image inversion interferometry.
11 Ιουν 2020 · Fluorescence microscopy has become a ubiquitous method to observe the location of specific molecular components within cells. However, the resolution of light microscopy is limited by the laws of diffraction to a few hundred nanometers, blurring most cellular details.
1 Ιαν 2014 · Resolution of a microscope is defined as the smallest distance at which two point-like objects could be discriminated. Optically, the resolution that a microscope can offer is limited by diffraction. Diffraction can be defined as the spreading of light waves as a result of their interaction with an object.
15 Μαΐ 2011 · Any microscopy technique that overcomes the resolution limit of conventional light microscopy by at least a factor of two is considered to be a super-resolution technique. Super-resolution techniques break the diffraction limit by temporally or spatially modulating the excitation or activation light.
6 Ιουλ 2018 · How do we determine a range of useful magnification for digital microscopy when an image is observed on a monitor? First, it is important to understand the resolution or resolving power of the microscope system and the viewing distance from the monitor.
9 Φεβ 2017 · The Abbe resolution limit is a sharp limit to the imaging of a periodic object such as a grating. Super-resolution refers to overcoming this resolution limit. The Rayleigh resolution criterion refers to imaging of a two-point object.
