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It involves a thermal cycler and three repeating steps: • Denaturation - DNA is heated to separate strands • Annealing – Primers are introduced to designate copying points • Elongation - Taq polymerase* synthesises new strand 30 cycles will produce over 1 billion copies of desired DNA sequence
The Central Dogma: DNA RNA Protein . 1. The following is a sequence of DNA. Some bases are missing. Fill them in. G A T T A C A __ __ C T A A __ __ T T G . 2. The following is a sequence of DNA. All bases are missing. Fill them in. C T T C C G C A A
What is the first step in eukaryotic DNA replication? The strands of the double helix separate, or unzip. 3. If the base sequence on a separated DNA strand is CGTAGG, what will the base sequence on its complementary strand be? The complementary strand will be GCATCC. 4. What enzyme joins individual nucleotides to produce the new strand of DNA?
Sequencing DNA requires a series of steps. Place the following steps in the correct sequence. The first and last ones have been done for you. A place sections in order by matching overlapping regions B cut DNA into sections of varying length C sequence short sections of DNA D amplify the DNA (create many copies) E extract samples of DNA from cells
27 Φεβ 2011 · Explain how molecular sequences, such as DNA, can be used to study evolutionary relationships. Summarize the process and goals of DNA sequence alignment. Interpret a simple phylogenetic tree.
Step 1a: Fill in the Nucleotides. Your instructor will give you two sheets, each with four nucleotides. Fill in each nucleotide component with a different color or pattern (polka dots, lines, etc.) so that it’s easy to tell them apart. The table below provides more information about each nucleotide component, including suggested colors.
After completing this, you would like to take a closer look at the sequence of nucleotides in the DNA fragment. You use a method called dideoxy Sanger sequencing. In this worksheet you will mimic the Sanger sequencing method to see how DNA is sequenced.
