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  1. EMB agar is used in water quality tests to distinguish coliforms and fecal coliforms that signal possible pathogenic microorganism contamination in water samples.

  2. 4 Ιαν 2022 · EMB Agar (Eosin Methylene Blue Agar) is recommended for the isolation and differentiation of gram negative enteric bacteria from clinical and nonclinical specimens. It is useful in differentiating gram positive and gram-negative bacteria.

  3. 1 Σεπ 2007 · EMB agar is used in water quality tests to distinguish coliforms and fecal coliforms that signal possible pathogenic microorganism contamination in water samples.

  4. 7 Μαρ 2022 · The eosin methylene blue agar (EMB agar) is a selective staining for gram-negative bacteria. It contains two dyes in the ratio of 6:1 and is both toxic to gram-positive microorganisms. It is both a selective and differential mediums for coliforms.

  5. Eosin Methylene Blue agar (EMB agar) is both selective and differential culture medium ideally used for isolating faecal coliforms. It is a selective medium due to inhibition of gram-positive bacteria by methylene blue. EMB agar’s differential property comes from the eosin and methylene blue complex formation under acidic pH.

  6. EMB Agar is recommended for the isolation and differentiation of gram negative enteric bacteria from clinical and nonclinical specimens. Methylene blue and Eosin-Y inhibit gram positive bacteria. The ratio of eosin and methylene blue is adjusted to 6:1.

  7. PRINCIPLE. Eosin and methylene blue dyes provide for differentiation of enteric gram-negative bacilli based the fermentation of lactose and the absorption of the dyes by the bacterial colonies. Eosin dye combines with methylene blue indicator to produce a color change when lactose is fermented.

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