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EMB Agar is used for the isolation of fecal coliforms. EMB Agar can be streaked for isolation or used in the Membrane Filter Technique. EMB agar inoculated with Escherichia coli demonstrates growth with green-metallic sheen colonies.
EMB agar is used in water quality tests to distinguish coliforms and fecal coliforms that signal possible pathogenic microorganism contamination in water samples.
4 Ιαν 2022 · Eosin Methylene Blue (EMB) agar is a differential microbiological medium, which slightly inhibits the growth of Gram-positive bacteria and provides a color indicator distinguishing between organisms that ferment lactose (e.g., E. coli) and those that do not (e.g., Salmonella, Shigella).
EMB Agar is recommended for the isolation and differentiation of gram negative enteric bacteria from clinical and nonclinical specimens. Methylene blue and Eosin-Y inhibit gram positive bacteria. The ratio of eosin and methylene blue is adjusted to 6:1.
Technical Data. EMB Agar M317. Intended Use: For the isolation and differentiation of Gram-negative enteric bacteria from clinical and non-clinical specimens. Composition** **Formula adjusted, standardized to suit performance parameters. Directions. distilled water. Mix until suspe. sion is uniform. Heat to boiling to dissolve the m.
EMB Agar (Eosin Methylene Blue Agar) is recommended for the isolation and differentiation of gram negative enteric bacteria from clinical and nonclinical specimens. Composition**
recommended that a nonselective, differential medium (MacConkey Agar or Hekton Enteric Agar) and a selective medium (Bismuth Sulfite Agar, SS Agar or Deoxycholate Citrate Agar) be run in parallel with EMB Agar.