Αποτελέσματα Αναζήτησης
Schematic drawing illustrating examples of genome editing assays in plants. The CRISPR/Cas9 technology was successfully applied in model plants (Nicotiana benthamiana, Arabidopsis thaliana) and crops (rice, wheat).The Cas9 nuclease and the sgRNA matching the gene of interest are co-expressed using Agrobacterium tumefaciens as a vector in N. benthamiana leaves or transfected into protoplasts ...
For example, genes that encode proteins capable of breaking down mycotoxins (Karlovsky, 2011) or inhibiting the activity of cell-wall-degrading enzymes can be introduced into plants. Plants can be engineered to synthesize and secrete antimicrobial peptides or compounds that directly inhibit colonization ( Osusky et al., 2000 ).
The current review describes various genome editing techniques and how they function, particularly CRISPR/Cas9 systems, which can contribute significantly to the most accurate characterization of genomic rearrangement and plant gene functions as well as the enhancement of critical traits in field crops.
25 Μαΐ 2020 · The results indicates that multiplex genome editing using CRISPR/Cas9 can be used to mimic the domestication process during evolution in a short time frame, with implications for a rapid and convenient generation of new plants with desirable traits.
Abstract. The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9 (Cas9) genome editing system is a powerful tool for targeted gene modifications in a wide range of species, including plants.
3 Ιουλ 2017 · The simplified approach to gene/genome editing represents a valuable tool for plant researchers in functional analysis of gene(s) and for breeders in the integration of key genes in the genomes of agriculturally important crops.
Developing GE elite plants carrying targeted gene mutation(s) without foreign DNA may help increase public acceptance of agricultural products and free them from regulatory monitoring in order to advance their use in plant breeding programs and commercial-scale production.
