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1 Αυγ 1999 · It has been successfully applied in the gene therapy of some single gene genopathy and plant genetical modification. The principle, examples and prospect of this technique was described.
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It has been successfully applied in the gene therapy of some...
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3 Ιουλ 2017 · Examples include targeted inactivation of endogenous genes in Arabidopsis [15, 16], high frequency modification of tobacco genes , and precise targeted addition of a herbicide-tolerance gene as well as insertional disruption of a target locus in maize .
23 Σεπ 2021 · Maher et al. describes a non-tissue culture method for the generation of genome edited plants through de novo induction of meristems using delivery of developmental regulators and gene editing reagents in somatic cells of whole plants.
With the emergence of new CRISPR-based tools, targeted gene editing has become increasingly efficient and flexible in plant cells. In addition to targeted gene mutagenesis, other types of genetic modifications, such as base substitution, gene knock-in and replacement, have become achievable in multiple plant species (Tables 1 and 2). New ...
24 Απρ 2017 · This proof-of-concept study demonstrated that it is possible to replace a wild-type gene with an altered one using CRISPR-Cas9 in plant cells. A year later, germline editing of the ADH1 gene was demonstrated in Arabidopsis [ 86 ].
When delivered into plant cells, SSN locate and cleave the target DNA, resulting in double-strand breaks (DSBs) that are repaired by natural DNA repair mechanisms like non-homologous end-joining (NHEJ) and homology-directed repair (HDR).
4 Ιουν 2022 · CRISPR and TALENs are efficient systems for gene editing in many organisms including plants. In many cases the CRISPR–Cas or TALEN modules are expressed in the plant cell only transiently.
