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25 Μαΐ 2020 · The results indicates that multiplex genome editing using CRISPR/Cas9 can be used to mimic the domestication process during evolution in a short time frame, with implications for a rapid and convenient generation of new plants with desirable traits.
3 Ιουλ 2017 · During the past few years, several novel genome editing systems have been developed; these include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9).
With the emergence of new CRISPR-based tools, targeted gene editing has become increasingly efficient and flexible in plant cells. In addition to targeted gene mutagenesis, other types of genetic modifications, such as base substitution, gene knock-in and replacement, have become achievable in multiple plant species (Tables 1 and 2). New ...
For example, genes that encode proteins capable of breaking down mycotoxins (Karlovsky, 2011) or inhibiting the activity of cell-wall-degrading enzymes can be introduced into plants. Plants can be engineered to synthesize and secrete antimicrobial peptides or compounds that directly inhibit colonization ( Osusky et al., 2000 ).
An example of modern methods of simple selection is marker-assisted selection, which uses molecular analysis to detect plants likely to express desired features, such as disease resistance to one or more specific pathogens in a population.
10 Μαρ 2023 · Main approaches used to create stable transgenic plants include (i) biolistic bombardment of plant cells and tissues with DNA coated nanoparticles using a gene gun, and (ii) Agrobacterium mediated transformation.
Increased production of plant growth hormones like auxin, cytokinin, and gibberellic acid, as well as root exudates like metallothionines and phytochelatins, may be the result of CRISPR-mediated gene expression in plants.
