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5 Αυγ 2022 · Electrophoresis is the migration of electrically charged molecules under the effect of the electrical field. Tiselius used a liquid medium that had less resolution due to the effect of gravity and diffusion. Electrophoresis uses solid support media with buffers to overcome these obstacles.
5 Δεκ 2022 · Sucrose concentration is a biomarker for biochemical efficiency in plants because it is involved in numerous metabolic processes associated with growth and development, control, and signaling [11]. However, the mechanisms in which sucrose participates as a signaling molecule remain widely unknown [10, 12].
1 Ιαν 2023 · Electrophoresis refers to the separation of charged molecules based on their mobility in an electric field. This is a routinely used technique employed for various preparative and analytical purposes including separation, purification, and characterization of nucleic acids (DNA and RNA) and proteins.
The electrophoresis system introduced here meets the requisites we had fixed to it. It allows to analyze easily proteins from 5 to 250 kDa with a single electrode buffer, various gels pH and minimally varying acrylamide concentrations.
25 Ιουλ 2023 · To minimize the diffusional mixing of materials being separated during electrophoresis, soluble gradient electrophoresis systems use non-ionic solute, for example, sucrose or glycerol (Fig. 20.1). Fig. 20.1. A solution electrophoresis system. Full size image.
28 Δεκ 2017 · In this work, a new spectrophotometric method for the determination of sucrose concentration in a pure and impure system was demonstrated. The calibration equation established was validated and compared with the refractometric method.
electrophoresis systems use varying densities of a 'non-ionic solute (e.g., sucrose or glycerol) to min- imize diffusional mixing of the materials being separated during electrophoresis (Fig. 4-1). Even with these refinements, solution electrophoresis systems have only limited application, usually when preparative scale electrophoretic separation
