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Introduction. This lab will determine the presence or absence of amplified DNA in your samples by visualization on an agarose gel. Arthropod and Wolbachia DNA, if present, will be distinguishable based on the size, or base pair (bp) length, of the DNA molecule.
Gel Electrophoresis Basics Worksheet. 1. Evaluate the following statements. Rewrite them so that they are correct if necessary. a) Each band in a DNA electrophoresis gel is made up of one molecule of DNA. b) Gel electrophoresis can tell you the sequence of a particular DNA fragment.
Gel Electrophoresis. Student Worksheet. Lab Number_____ Name ______________________. DNA fragment analysis. Draw the results from your gel on the diagram below. Identifying the genotype. Write in the genotype of the samples, then work out the genotype of the parents. T = Short Tail t = Long Tail. N.
Scientists most commonly use gel electrophoresis to separate DNA fragments. Because DNA is negatively charged, it moves towards the positive electrode in an electric field.
This document provides questions and instructions for a lab on gel electrophoresis. It defines micropipette volumes and proper pipetting techniques to avoid contaminating samples. It also describes the steps of running a gel electrophoresis, including making an agarose gel, loading DNA samples and a size ladder, and running the gel with an ...
Appendix A - What is Gel Electrophoresis? Looking at a sample of green dye, how can you know if it is really green? Could it be a mixture of blue and yellow dyes? Electrophoresis is a technique used in many areas of science to analyze and separate samples by applying a constant electric field.
GEL ELECTROPHORESIS CONCEPT QUESTIONS. 1. What is a marker or ladder? Why is this considered a standard in this lab? 2. Why is a ladder or standard necessary part of this lab? Why does each lab team need to run their own ladder or standard? 3. How does the size of the DNA fragment affect its movement or migration through the agarose gel during ...