Αποτελέσματα Αναζήτησης
15 Φεβ 2011 · Watch this JoVE video on The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds
1 Φεβ 2009 · In this video, we demonstrate each step of preparing sensory-motor neuron cultures, including anesthetizing adult and juvenile Aplysia, dissecting their ganglia, protease digestion of the...
This protocol describes the use of these growth factors and supplemental reagents for successful culture of motor neurons in vitro together with a simple step-by-step method for isolating and culturing these neurons.
1 Ιαν 2009 · We are particularly interested in neurodegenerative diseases and so altering cell death for the purposes of cell culture would defeat the object of the investigations. This chapter describes our principal sensory and motor neuron cell culture techniques.
19 Μαρ 2021 · In this protocol, we provide step-by-step instructions for dissection and culture of primary murine dorsal root ganglia (DRG), which provide an opportunity to study the functional properties of peripheral sensory neurons in vitro. Further, we describe the analysis of neuropeptide release by ELISA as a possible downstream application.
26 Οκτ 2019 · In the presented protocol, we explain how to isolate cerebellar neurons and maintain their viability with optimum survival in vitro for a maximum of 3 weeks. Primary culture of cerebellar cells, which were isolated at E15−E18, confirms the collection of three classes of large neurons: PCs, Golgi cells, and CNs.
19 Δεκ 2007 · Prepare one day prior to isolation under sterile conditions. Start with Poly-D-Lysine (BD cat no. 354210) 20 mg vial; reconstitute with 4 mL UltraPure Distilled water to 5mg/ml stock solution. Divide to 1 mL aliquots and store at -20°C.
