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1. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer designing tool - National Center for Biotechnology...

   www.ncbi.nlm.nih.gov › tools › primer-blast

   • Αποθηκευμένο αντίγραφο

   Primer-BLAST uses Primer3 and BLAST to design primers for PCR based on a template sequence. You can enter your own primers, set parameters for primer specificity, melting temperature, exon/intron selection, and search mode.

   • Primer-BLAST

     Maximum number of database sequences (with unique sequence...

   • Tips for Finding Specific Primers

     Things you can do to maximize the chance of finding primers...

   • Primer-BLAST Help

     Primer-BLAST Results Help. Primer-BLAST was developed at...

   • Blast

     The Basic Local Alignment Search Tool (BLAST) finds regions...

   • Publication

     BLAST and global alignment algorithm to screen primers...

   • PMC Free Article

     We present a new software tool called Primer-BLAST to...

2. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer-Blast results - National Center for Biotechnology...

   www.ncbi.nlm.nih.gov › tools › primer-blast

   • Αποθηκευμένο αντίγραφο

   Primer-BLAST Results Help. Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target. It uses Primer3 to design PCR primers and then uses BLAST and global alignment algorithm to screen primers against user-selected database in order to avoid primer pairs (all combinations including forward-reverse ...

3. blast.ncbi.nlm.nih.gov › BlastBasic Local Alignment Search Tool - BLAST

   blast.ncbi.nlm.nih.gov › Blast

   • Αποθηκευμένο αντίγραφο

   The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

4. www.ncbi.nlm.nih.gov › tools › primer-blastNational Center for Biotechnology Information

   www.ncbi.nlm.nih.gov › tools › primer-blast

   • Αποθηκευμένο αντίγραφο

   BLAST and global alignment algorithm to screen primers against user-selected database. in order to avoid primer pairs (all combinations including forward-reverse primer pair, forward-forward as well as reverse-reverse pairs) that can cause non-specific amplifications.

5. www.ncbi.nlm.nih.gov › guide › howtoDesign PCR primers and check them for specificity - National...

   www.ncbi.nlm.nih.gov › guide › howto

   • Αποθηκευμένο αντίγραφο

   Primer BLAST performs only a specificity check when a target template and both primers are provided. In the Primer Pair Specificity Checking Parameters section, select the appropriate source Organism and the smallest Database that is likely to contain the target sequence.

6. www.ncbi.nlm.nih.gov › pmc › articlesPrimer-BLAST: A tool to design target-specific primers for...

   www.ncbi.nlm.nih.gov › pmc › articles

   18 Ιουν 2012 · We present a new software tool called Primer-BLAST to alleviate the difficulty in designing target-specific primers. This tool combines BLAST with a global alignment algorithm to ensure a full primer-target alignment and is sensitive enough to detect targets that have a significant number of mismatches to primers.

7. www.ncbi.nlm.nih.gov › tools › primer-blastNational Center for Biotechnology Information

   www.ncbi.nlm.nih.gov › tools › primer-blast

   • Αποθηκευμένο αντίγραφο

   Things you can do to maximize the chance of finding primers specific for your template. Use refseq accession or GI (rather than the raw DNA sequence) as template whenever possible.