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Pierce HRV 3C Protease cleaves protein substrates with the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro between the Gln and Gly residues. The high specificity and dual affinity tags (GST and 6xHis) of the protease make it an ideal choice for the removal of purification and detection tags on recombinant
10 Ιαν 2021 · GST (Antoniou et al., 2017) and the biotin acceptor peptide (BAP) (Youell et al., 2011) have been proposed and shown to enhance the production of soluble HRV 3C protease. Yields of 22 mg/L and 25−30 mg/L cell culture were reported for HRV 3C protease tagged with GST or BAP, respectively.
PreScission Protease is a fusion protein of human rhinovirus (HRV) 3C protease and GST. It allows for on-column cleavage of GST tags and protein purification in one step. Specific cleavage – between the Gln and Gly residues of the recognition sequence LeuGluValLeuPheGln/GlyPro.
The Glutathione S-transferase (GST) Gene Fusion System from Cytiva is a versatile system for the expression, purification, and detection of GST-tagged proteins produced in E. coli. The system consists of three major components: pGEX plasmid expression vectors, products for GST purification, and a variety of GST detection products.
Map of the GST vectors showing the reading frames and main features. All 13 vectors have stop codons in all three reading frames downstream from the MCS (not depicted in this map). See Appendix 2 (Control regions for pGEX vectors) for the control regions of the 13 vectors.
High affinity purification of the GST-tagged fusion proteins expressed using pCold GST is possible. Furthermore, a highly specific HRV 3C protease recognition sequence has been inserted between the GST tag and MCS, allowing removal of the GST tag from the recombinant protein. Because the optimum reaction temperature for HRV 3C protease is low
MCLAB's HRC 3C Protease is fused to GST, so that the fusion tag can be easily removed from the protein by cleavage reactions using immobilized glutathione (GSH). HRV 3C Protease is highly active at 4°C, and also active in a variety of commonly used buffers, providing more flexibility in experimental design to keep the activity
