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  1. EMBOSS Needle reads two input sequences and writes their optimal global sequence alignment to file. You can choose the sequence type, output format, matrix, gap parameters and more options for your analysis.

    • Job Dispatcher

      The Job Dispatcher at EMBL-EBI offers free access to a range...

  2. Pairwise Structure Alignment. This tool allows the selection of protein 3D structures for alignment. Use an existing PDB or Computed Structure Model entry ID, upload a local file with atomic coordinates, or enter a URL of a file on the web.

  3. Clustal Omega is a tool for aligning three or more sequences using seeded guide trees and HMM profile-profile techniques. For pairwise sequence alignment, use the example sequence or paste your own sequence and select the sequence type (protein, DNA, RNA).

  4. Job Dispatcher offers free access to various bioinformatics tools and datasets, including pairwise sequence alignment tools. You can identify regions of similarity between two biological sequences using Needle, Stretcher, GGEARCH2SEQ, Water, Matcher and more.

  5. 23 Σεπ 2024 · The structure alignment tool provides a simple-to-use, web-accessible interface for performing a wide range of structural superpositions. The tool can be accessed from the “Analyze” section of the menu bar. The interface allows you to align one or more structures to a given reference structure.

  6. CLUSTALW is a tool for aligning multiple sequences of DNA or protein. It offers different parameters and options for pairwise and multiple alignment algorithms, as well as output formats and weight matrices.

  7. SIM - Alignment Tool for Protein Sequences. SIM is a program which finds a user-defined number of best non-intersecting alignments between two protein sequences or within a sequence [more]. Once the alignment is computed, you can view it using LALNVIEW, a graphical viewer program for pairwise alignments [reference to LANVIEW].

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