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A 0.9% NaCl solution is said to be isotonic: when blood cells reside in such a medium, the intracellular and extracellular fluids are in osmotic equilibrium across the cell membrane, and there is no net influx or efflux of water.
- Procedure
Six labelled centrifuge tubes are prepared containing 10 ml...
- Procedure
9 Αυγ 2024 · 0.9% NaCl (Normal Saline Solution, NSS) Normal saline solution (0.9% NaCl) or NSS, is a crystalloid isotonic IV fluid that contains water, sodium (154 mEq/L), and chloride (154 mEq/L). It has an osmolality of 308 mOsm/L and gives no calories.
Label the three other panels by indicating which shows RBCs added to 0.09% NaCl, to 0.9% NaCl, and to 9% NaCl. Below the panels, indicate whether each solution is isotonic, hypertonic, or hypotonic in relation to RBCs.
Typical isotonic solutions are 0.9% m/v NaCl solution in water or 5% m/v glucose solution in water. Red blood cells placed in a hypertonic solution shrink in size due to more flow of water out than into the cell –a process called crenation.
When placed in a hypertonic solution, a red blood cell will lose water and undergo crenation (shrivel). Animal cells tend to do best in an isotonic environment, where the flow of water in and out of the cell is occurring at equal rates.
19 Μαΐ 2017 · To calculate the osmolarity, given that NaCl dissociates into two ions (Na + and Cl −) in solution and has an osmotic coefficient of 0.93, the following equation is used: Osmolarity of solution ( mosM ) = molarity ( M ) × number of osmoles produced by dissociation × osmotic coefficient
21 Μαρ 2023 · First, we know that a 0.9% NaCl solution is considered isotonic to RBCs, meaning that the concentration of solutes inside and outside the cell is balanced, and there is no net movement of water in or out of the cell.
