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σχετικά με: reverse transcriptase cdna ab results normal valuesHigh-performance first-strand cDNA for many applications including PCR and qPCR. Superior enzyme for all RNAs, including difficult templates. Bulk pricing available.
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Reverse transcriptases are essential for synthesizing complementary DNA (cDNA) strands from RNA templates. A deeper understanding of the properties of reverse transcriptases and their implications in cDNA synthesis is vital to the success of molecular biology experiments.
21 Μαΐ 2018 · In this paper, we present a method of quantitative PCR normalization using the total amount of cDNA generated during RT. Our approach controls for the amount of starting material and variation of transcription efficiencies across RT reactions.
Several published articles have addressed this question by attempting to put a value on RNA-to-cDNA conversion efficiency, yet results vary widely with different experimental conditions. Some cited efficiency ranges are 49–114% , 50–77% , 0–102% , and 39–65% . This wide variety effectively illustrates the problem and is likely a ...
RT-qPCR is a powerful tool for RNA analysis. It applies the power of PCR to an RNA sample by first reverse transcribing RNA into cDNA, then amplifying this cDNA template using PCR. RNA template quantity and purity and efficient reverse transcription are all important for successful RT-qPCR.
ProtoScript® II Reverse Transcriptase (NEB #M0368) is capable of generating cDNA of more than 10kb, at temperatures up to 48°C. We recommend 42°C for routine reverse transcription. You can increase the yield of long cDNA products by doubling the amount of enzyme and dNTP.
Induro ® Reverse Transcriptase Fast, processive, intron-encoded RT Ideal for long transcripts, direct RNA sequencing, cDNA synthesis from samples with strong secondary structures and/or with inhibitors
15 Ιουλ 2020 · Reverse transcription-PCR (RT-PCR) is a common laboratory technique that uses reverse transcriptase (RT) to create cDNA from target RNA sequences, which are amplified via the PCR. RT-PCR is either performed as a two-step or one-step assay.
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σχετικά με: reverse transcriptase cdna ab results normal valuesHigh-performance first-strand cDNA for many applications including PCR and qPCR. Superior enzyme for all RNAs, including difficult templates. Bulk pricing available.
