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We describe a method to facilitate electrophoretic separation of high molecular weight RNA species, such as ribosomal RNAs and their precursors, on agarose-formaldehyde gels.
- Denaturation and electrophoresis of RNA with formaldehyde
Electrophoretic size fractionation can be used to denature...
- Separation of RNA according to Size: Electrophoresis of RNA through ...
Samples of RNA may be denatured by treatment with formamide...
- Denaturation and electrophoresis of RNA with formaldehyde
2 Φεβ 2015 · Electrophoretic size fractionation can be used to denature and separate large mRNA molecules (0.5-10 kb) on formaldehyde-containing agarose gels. Formaldehyde contains a carbonyl group that reacts to form Schiff bases with the imino or amino groups of guanine, adenine, and cytosine.
To 1-3 µg RNA, add 0.5-3X volumes Formaldehyde Load Dye. To simply check the RNA on a denaturing gel, as little as 0.5X Formaldehyde Load Dye can be used, but to completely denaturate the RNA, e.g. for Northern blots, use 3X volumes of Formaldehyde Load Dye.
Electrophoretic size fractionation can be used to denature and separate large mRNA molecules (0.5–10 kb) on formaldehyde-containing agarose gels. Formaldehyde contains a carbonyl group that reacts to form Schiff bases with the imino or amino groups of guanine, adenine, and cytosine.
The methodology described in this chapter involves the use of formaldehyde as a denaturant within the agarose gel. In addition, both formaldehyde and formamide are added to the sample before electrophoresis to aid the denaturation of the RNA sample.
1 Οκτ 2013 · We describe a method to facilitate electrophoretic separation of high-molecular-weight RNA species, such as ribosomal RNAs and their precursors, on agarose–formaldehyde gels. Two alternative “p K -matched” buffer systems were substituted for the traditionally used Mops-based conductive medium.
1 Φεβ 2022 · Samples of RNA may be denatured by treatment with formamide and separated by electrophoresis through agarose gels containing formaldehyde. In this method, RNA is fractionated by electrophoresis through an agarose gel containing 2.2 m formaldehyde.
