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The slide test is performed first to detect bound coagulase, since it is a very quick procedure, and only a negative strain is further tested in the tube coagulase test.
18 Ιαν 2022 · Procedure of Coagulase Test. Coagulase can be detected by two different methods; tube test and slide test. Slide Test. About 10 µl of deionized water or physiological saline is added to a slide. Several colonies from a fresh culture are collected with an inoculating loop and are emulsified into the water to obtain a smooth milk-colored suspension.
23 Νοε 2019 · It describes enzyme-based tests like catalase, coagulase, pyrrolidonyl arylamidase (PYR), and oxidase that examine single enzymes or metabolic pathways. It also covers tests that analyze an isolate's ability to grow in the presence of inhibitors like antibiotics, salts, and surfactants.
10 Αυγ 2022 · Slide Test (to detect bound coagulase) Place a drop of physiological saline on each end of a slide, or on two separate slides. With the loop, straight wire or wodden stick, emulsify a portion of the isolated colony in each drops to make two thick suspensions.
This document describes the slide coagulase test and tube coagulase test used to identify Staphylococcus aureus. The slide coagulase test detects bound coagulase, which causes bacterial clumping. The tube coagulase test detects free coagulase secreted by S. aureus, which reacts with plasma to form a clot.
• Understand the biochemical basis of the coagulase test; • Properly perform both the tube and slide coagulase tests; and • Discern how this information can be used to differentiate staphylococcal species.
Description: provides a detailed explanation of the purpose, mechanism, method, and interpretation of the coagulase test. Steps: step-by-step instructions for carrying out the coagulase test, with short video clips demonstrating each step. Click on the H2O bottle and drag it to a glass slide to place a drop of H2O on the slide.
