Αποτελέσματα Αναζήτησης
19 Αυγ 2019 · Inorganic polyphosphates (polyPs) and inositol pyrophosphates (PP-InsPs) form important stores of inorganic phosphate and can act as energy metabolites and signaling molecules. Here we review our current understanding of polyP and inositol phosphate (InsP) metabolism and physiology in plants.
16 Σεπ 2022 · Inorganic phosphate (Pi) and phosphorus (P) homeostasis are essential for plant growth and yield, and reliable detection of dynamic Pi/P in different tissues is important for studying their biological functions. Here, we report a combined protocol for rapid determination of Pi/P levels.
20 Ιουν 2022 · Plants acquire P almost exclusively by transporting soluble inorganic H 2 PO 4– (Pi) from the soil into their roots. The Pi concentration in most soil solutions ranges between 0.5 and 10 μM, whereas the optimal intracellular Pi concentration is 5–20 mM.
9 Ιαν 2024 · Here we develop a rapid colorimetric Pi imaging method, inorganic orthophosphate staining assay (IOSA), that can semi-quantitatively image intracellular Pi with high resolution.
26 Ιαν 2015 · Fluorescence indicator protein for inorganic phosphate (FLIPPi) sensors are genetically encoded fluorescence resonance energy transfer-based sensors that have been used to monitor Pi dynamics in cultured animal cells. Here, we present a series of Pi sensors optimized for use in plants.
21 Φεβ 2022 · In plant tissues, phosphorus occurs in organic forms such as phosphate monoester (C-O-P bond) (e.g., phytate), phosphate diesters (C–O–P–O–C bond) (e.g., nucleotides and phospholipids), and inorganic forms such as orthophosphate, pyrophosphate (P-O-P bond), and polyphosphate.
Here we use a polyP-specific dye and a polyP-binding domain to detect polyPs in plant and algal cells. To develop the staining protocol, we induced polyP granules in Nicotiana benthamiana and Arabidopsis cells by heterologous expression of Escherichia coli polyphosphate kinase 1 (PPK1).
