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HTRF™ (Homogeneous Time Resolved Fluorescence) is a no-wash technology that combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence.
28 Μαΐ 2009 · HTRF (Homogeneous Time Resolved Fluorescence) is the most frequently used generic assay technology to measure analytes in a homogenous format, which is the ideal platform used for drug target studies in high-throughput screening (HTS). This technology combines fluorescence resonance energy transfer …
HTRF principles z Fluorescence resonance energy transfer z Principles of TR-FRET z HTRF donor and acceptor fluorophores z FAQs HTRF detection z Ratiometric measurement
16 Ιουν 2020 · HTRF technology. Four specific fluorophores, forming different time-resolved FRET couples, are used in HTRF. The donor lanthanide fluorophore is either europium (Eu3+) or terbium (Tb2+) cryptate (fig. 1), whereas the steady-state fluorescence acceptors usually emit in the red range.
Time-Resolved Fluorescence (TRF) and TR-FRET (HTRF) are the powerful tools for drug discovery which enable the analysis of molecular interactions in biochemical processes like kinase assays, cellular signaling pathways, protein-protein interactions.
17 Αυγ 2022 · 1 INTRODUCTION. Epidermal growth factor receptor (EGFR) is a cell-surface receptor and plays a key role in understanding the physiological signaling of receptor tyrosine kinases (Truong et al., 2016).It has been known that EGFR can promote cells into uncontrolled proliferation and a continuous dividing state, which leads to the malignant progression of tumor cells (Kumar et al., 2021; Liang ...
