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  1. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

    • Blast

      The Basic Local Alignment Search Tool (BLAST) finds regions...

    • Home

      The Basic Local Alignment Search Tool (BLAST) finds regions...

    • Recent Results

      Enter a BLAST RID in the RID box below and click Go to find...

    • Saved Strategies

      clicking the 'Saved Search Strategy' link at the top of a...

    • Help

      Welcome to BLAST Help¶ Getting started¶ Web BLAST quick...

    • Nucleotide Blast

      PSI-BLAST allows the user to build a PSSM (position-specific...

    • Blastx

      PSI-BLAST allows the user to build a PSSM (position-specific...

    • NCBI

      The National Center for Biotechnology Information advances...

  2. Select the sequence database to run searches against. No BLAST database contains all the sequences at NCBI. BLAST databases are organized by informational content (nr, RefSeq, etc.) or by sequencing technique (WGS, EST, etc.). more...

  3. BLAST is a tool for comparing sequences from NCBI databases or user-submitted data. It can help identify homologous regions, functional domains, and evolutionary relationships.

  4. Learn how to choose the best BLAST program for your sequence analysis needs based on the query, database, program, and search goal. Compare the features and contents of different BLAST databases, including nucleotide, protein, genome, and specialized databases.

  5. Learn about the new features and enhancements of BLAST, the popular tool for sequence similarity search. Find links to BLAST forms, databases, documentation, and recent results.

  6. This video tutorial is an easy step-by-step guide for using the NCBI BLAST bioinformatics tool for your genomic research.

  7. BLAST is available on the web on the NCBI website. Different types of BLASTs are available according to the query sequences and the target databases. Alternative implementations include AB-BLAST (formerly known as WU-BLAST), FSA-BLAST (last updated in 2006), and ScalaBLAST.

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