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12 Σεπ 2021 · The chromatographic peak in Figure 12.2.9 a is an example of peak tailing, which occurs when some sites on the stationary phase retain the solute more strongly than other sites. Figure 12.2.9 b, which is an example of peak fronting most often is the result of overloading the column with sample.
31 Ιαν 2021 · The peak at 7.87 min has a height of 329451 but only approximately 20 V in the chromatogram. How can I calculate this height?
1 Δεκ 2017 · The top section of most chromatographic peaks is almost an ideal Gaussian shape-for example, the >80% height (Figure 1). To confirm this hypothesis, the standard deviation should be extracted at other heights (for example, 85%, 90%).
The most effective and convenient way to alter the retention factor of a peak is to adjust the ‘solvent strength’ of the chromatographic mobile phase. This is usually achieved in reversed phase chromatography by changing the amount of organic solvent (modifier) in the mobile phase mixture.
1 Οκτ 2013 · Real chromatographic peaks have some degree of asymmetry; their front and back halves are not the same shape. Extra-column dead volumes, peak mass overloading, and reversible adsorption effects are some of the principal causes of peak asymmetry.
Good Peak Shape • Wide-pore 300Å totally porous columns can be selected for efficient peaks when separating proteins and peptides. • Select wide-pore columns for lower molecular weight molecules with large hydrodynamic volumes. • Select Poroshell columns for more rapid mass transfer and improved efficiency of large peptides and proteins
1 Ιουλ 2016 · The true measure of analyte quantity is peak area. The height of a peak indirectly relates to q via its relation to area. Nevertheless, height proves to be a better characteristic of analyte content than area when this latter cannot be measured accurately due to peak overlap, asymmetry, or poor S/N ratio [14], [43].
