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To simply check the RNA on a denaturing gel, as little as 0.5X Formaldehyde Load Dye can be used, but to completely denaturate the RNA, e.g. for Northern blots, use 3X volumes of Formaldehyde Load Dye. Ethidium bromide can be added to the Formaldehyde Load Dye at a final concentration of 10 µg/ml.
We describe a method to facilitate electrophoretic separation of high molecular weight RNA species, such as ribosomal RNAs and their precursors, on agarose-formaldehyde gels.
2 Φεβ 2015 · Electrophoretic size fractionation can be used to denature and separate large mRNA molecules (0.5-10 kb) on formaldehyde-containing agarose gels. Formaldehyde contains a carbonyl group that reacts to form Schiff bases with the imino or amino groups of guanine, adenine, and cytosine.
Electrophoretic size fractionation can be used to denature and separate large mRNA molecules (0.5–10 kb) on formaldehyde-containing agarose gels. Formaldehyde contains a carbonyl group that reacts to form Schiff bases with the imino or amino groups of guanine, adenine, and cytosine.
1 Οκτ 2013 · We describe a method to facilitate electrophoretic separation of high-molecular-weight RNA species, such as ribosomal RNAs and their precursors, on agarose–formaldehyde gels. Two alternative “p K -matched” buffer systems were substituted for the traditionally used Mops-based conductive medium.
gels (formaldehyde-agarose or polyacrylamide-urea) reduce effects of RNA secondary structure and provide more accurate size determinations than native gels, but are difficult to run and stain. Denaturing gels are
RNA has the tendency to form both secondary and tertiary structures that can Impede its separation by electrophoresis. As such, identical species of RNA exhibiting varying degrees of intramolecular base-pairing, migrate at different rates and result in the smearing of distinct RNA molecules.
