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10 Αυγ 2022 · Some sugars such as glucose are called reducing sugars because they are capable of transferring hydrogens (electrons) to other compounds, a process called reduction. When reducing sugars are mixed with Benedicts reagent and heated, a reduction reaction causes the Benedicts reagent to change color.
Benedict’s test is a chemical test that can be used to check for the presence of reducing sugars in a given analyte. Therefore, simple carbohydrates containing a free ketone or aldehyde functional group can be identified with this test.
Benedict's reagent (often called Benedict's qualitative solution or Benedict's solution) is a chemical reagent and complex mixture of sodium carbonate, sodium citrate, and copper(II) sulfate pentahydrate. [1] It is often used in place of Fehling's solution to detect the presence of reducing sugars and other reducing substances. [2]
Benedict’s reagent is the solution used in Benedict’s test to detect simple sugars such as glucose. It is a bright blue solution prepared by mixing copper sulfate pentahydrate (CuSO 4. 5H 2 O), sodium citrate (Na 3 C 6 H 5 O 7), and sodium carbonate (Na 2 CO 3) in distilled water [4].
19 Απρ 2002 · Benedict's Solution, or one of the many variants that evolved over the years, was used as the reagent of choice for measuring sugar content for more than 50 years. It was the most common test for diabetes and was the standard procedure for virtually all clinical laboratories.
9 Μαρ 2022 · Principle of Benedict’s Test. Sodium carbonate in the Benedict reagent increases the pH of the sample-reagent solution mixture. Under warm alkaline conditions reducing sugars are tautomerism to strong reducing agents, enediols.
4 Μαΐ 2022 · Benedict’s test is based on the principle that under alkaline conditions reducing sugar forms enediols which are powerful reducing agents. The benedict solution contains milder alkali, Na 2 CO 3 to maintain alkaline conditions. Reducing sugar which is changed into enediols reduces the cupric ions to cuprous ions.
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