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21 Ιαν 2016 · Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) is a method that allows the study of protein complexes, in particular chromatin and transcription factor complexes, in a...
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HEPES-NaOH – HEPES-NaOH is an organic chemical buffer solution that works best between pH 7.2 and 8.2. It is ideally used in cases where enzyme structure and function need to be maintained at low temperatures.
6 Ιουν 2022 · Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) is a technique to study protein complexes on chromatin. The protocol below describes specific steps for RIME analysis of the male human-derived prostate cancer cell line LNCaP.
1 Νοε 2020 · Because of the great importance of precipitation methods for the processing of samples in proteomics, the acetone, methanol-chloroform (M/C), and trichloroacetic acid (TCA)-acetone protocols were compared for CHO cells in terms of protein recovery, band pattern resolution, and presence on SDS-PAGE. Results.
Among the buffering ion screening (Figure 3a), HEPES and Tris up to 0.1 M gave a less than 20% reduction in tryptic activity. Equally, the chaotrope octylglucoside (Figure 3d) showed to have only a small impact on the digestion eficiency; the effect was not found to be concentration-dependent.
1 Δεκ 2017 · Here, we performed an iterative and comprehensive evaluation of digestion conditions for five structurally diverse proteins in plasma and serum: apolipoprotein A-1, retinol-binding protein 4, transthyretin, complement component 9 and C-reactive protein.
31 Οκτ 2018 · Abstract. Chemical cross-linking in combination with mass spectrometric analysis of the created cross-linked products is an emerging technology aimed at deriving valuable structural information...
