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22 Ιουν 2020 · Introduction. Chromosome replication in eukaryotes is performed by three different B-family DNA polymerases (Pol), Pol ε, Pol δ, and Pol α-primase 1, 2, 3, 4. Pol ε performs bulk leading...
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6 Σεπ 2017 · It has been shown that the A-family polymerase KlenTaq (a Klenow-fragment analogue of Taq polymerase) follows the A rule by using the side chain of Tyr671 to bind incoming dATP 173.
8 Αυγ 2017 · Here, we propose a translocation mechanism that points to the flexibility of the DNA, including its overstretching transition, as the principal responsible for the DNA polymerase ratcheting...
1 Σεπ 2015 · In brief, a D598A/F710Y double mutant DNA polymerase I from BF large fragment was mixed with excess annealed oligonucleotides (template 5′-GAGCGAGCATCATG-3′ and primer 5′-CATGATGC-3′) and 1 mM ddTTP, which prevents incorporation of more nucleotides into the primer chain.
The process of homologous recombination involves the following three major steps: the search for the right fragments of the partner DNA molecule (homology recognition), exchange of the DNA strands between the pair of DNA molecules (strand exchange), and resolution of the branched DNA structure (branch resolution).
1 Ιαν 2014 · As a polymeric material with known nanometer-scale dimensions and well-understood, programmable, molecular recognition capabilities, DNA has become a leading construction material for bottom-up fabrication of nanomaterials with complex structures and functions.
Taking advantage of recent advances in cryo-EM, we present here a cryo-EM structure of the complete yeast Polδ holoenzyme in the act of DNA synthesis, at a nominal resolution of 3.2 Å ( Fig. 1b ).
