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Fractionation of Cells. Although biochemical analysis requires disruption of the anatomy of the cell, gentle fractionation techniques have been devised to separate the various cell components while preserving their individual functions.
1 Ιαν 2017 · PDF | On Jan 1, 2017, Melissa Petiti and others published Cell Fractionation | Find, read and cite all the research you need on ResearchGate.
In this chapter, we describe a simple and efficient method to isolate the different compartments of Escherichia coli by a fractionation method and to determine the presence of the protein of interest. For inner membrane proteins, we propose a method to discriminate between integral and peripheral membrane proteins.
1 Φεβ 2011 · For providing evidence for the most reliable and efficient separation technique, we compared four different procedures for subcellular fractionation of Jurkat cells.
17 Αυγ 2007 · This chapter presents techniques for the fractionation of cellular components, including organelles and appendages, beginning with external surfaces and ending with internal components.
FRACTIONATION. After homogenization, the cellular components need to be separated/fractionated. Again, the pro-tocols in this manual, for the most part, use the same technique: differential centrifugation. During centrifugation, cellular components are pelleted based on size, density, and sedimentation properties.
Four great things about subfractionation: Study single biological processes free from other interfering reactions in the cell w/o having to worry about keeping the cells alive-cell free system. Fractions are biologically active and can be stored. Minimal equipment needed and starting material is easily obtained.
