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1 Φεβ 2011 · In order to maximize coverage in proteome studies, a successful approach is the fractionation of cellular compartments. For providing evidence for the most reliable and efficient separation ...
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In order to maximize coverage in proteome studies, a...
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1 Ιαν 2017 · PDF | On Jan 1, 2017, Melissa Petiti and others published Cell Fractionation | Find, read and cite all the research you need on ResearchGate
17 Αυγ 2007 · This chapter presents techniques for the fractionation of cellular components, including organelles and appendages, beginning with external surfaces and ending with internal components.
Populations of cells can be analyzed biochemically by disrupting them and fractionating their contents by ultracentrifugation. Further fractionations allow functional cell-free systems to be developed; such systems are required to determine the molecular details of complex cellular processes.
1 Ιαν 2014 · Fractionation of the cells into subcellular compartments enables protein enrichment and is essential to accurately determine the localization of specific proteins, which is the first step towards understanding the function of a protein in the cell. Download protocol PDF.
15 Μαΐ 2001 · Cell fractionation is a useful preparative and analytical method in cell biology. It is essential for analysis of composition and function of cellular compartments and it is used to prepare materials...
In this chapter, we describe a simple and efficient method to isolate the different compartments of Escherichia coli by a fractionation method and to determine the presence of the protein of interest. For inner membrane proteins, we propose a method to discriminate between integral and peripheral membrane proteins.
