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Populations of cells can be analyzed biochemically by disrupting them and fractionating their contents by ultracentrifugation. Further fractionations allow functional cell-free systems to be developed; such systems are required to determine the molecular details of complex cellular processes.
17 Αυγ 2007 · This chapter presents techniques for the fractionation of cellular components, including organelles and appendages, beginning with external surfaces and ending with internal components.
in Molecular and Cell Biology focuses on the application of modern centrifugation technology in molecular and cell biology including the separation and fractionation of biological particles by centrifugation on the preparative and analytical scales
1 Φεβ 2011 · In order to maximize coverage in proteome studies, a successful approach is the fractionation of cellular compartments. For providing evidence for the most reliable and efficient separation ...
1 Νοε 2017 · Cell fractionation allows for the individual study of organelles to better understand their biochemical functions and the molecular basis of diseases. The protocol involves homogenizing cells in buffer to break them open followed by differential centrifugation to separate organelles based on density.
Cellular fractionation. To distinguish nanoparticulate cargo in the cytosol from that sequestered in the endolysosomal compartment, a frequently used assay involves fractionation of the cells and measuring the cargo in the cytosolic and endosomal fractions with in vitro assays.
Fractionation of subcellular proteins enables protein localization assessment and protein enrichment from specific cellular compartments.
