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Populations of cells can be analyzed biochemically by disrupting them and fractionating their contents by ultracentrifugation. Further fractionations allow functional cell-free systems to be developed; such systems are required to determine the molecular details of complex cellular processes.
17 Αυγ 2007 · This chapter presents techniques for the fractionation of cellular components, including organelles and appendages, beginning with external surfaces and ending with internal components.
1 Ιαν 2017 · The simple two-step method involving resuspension of the cells in a fractionation buffer, followed by recovery of the periplasmic fraction, has been shown to be feasible for use in both the...
Cellular fractionation. To distinguish nanoparticulate cargo in the cytosol from that sequestered in the endolysosomal compartment, a frequently used assay involves fractionation of the cells and measuring the cargo in the cytosolic and endosomal fractions with in vitro assays.
Fractionation of subcellular proteins enables protein localization assessment and protein enrichment from specific cellular compartments.
1 Φεβ 2011 · In order to maximize coverage in proteome studies, a successful approach is the fractionation of cellular compartments. For providing evidence for the most reliable and efficient separation ...
1 Ιουλ 2017 · Different approaches can be used to determine the protein localisation within a cell such as in silico identification of protein signal sequences and motifs, electron microscopy and immunogold labelling, optical fluorescence microscopy, and biochemical technics. In this chapter, we describe a simple and efficient method to isolate the different ...
