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Populations of cells can be analyzed biochemically by disrupting them and fractionating their contents by ultracentrifugation. Further fractionations allow functional cell-free systems to be developed; such systems are required to determine the molecular details of complex cellular processes.
Cellular fractionation. To distinguish nanoparticulate cargo in the cytosol from that sequestered in the endolysosomal compartment, a frequently used assay involves fractionation of the cells and measuring the cargo in the cytosolic and endosomal fractions with in vitro assays.
1 Φεβ 2011 · For providing evidence for the most reliable and efficient separation technique, we compared four different procedures for subcellular fractionation of Jurkat cells.
30 Ιαν 1997 · Basic separation techniques needed in all cell biology laboratories are given and presented in the context of a number of related topics such as the labelling of ligands, the kinetics of their internalization and analysis of data, and the electron microscopy of organelles.
17 Αυγ 2007 · This chapter presents techniques for the fractionation of cellular components, including organelles and appendages, beginning with external surfaces and ending with internal components.
In cell biology, cell fractionation is the process used to separate cellular components while preserving individual functions of each component. [1] This is a method that was originally used to demonstrate the cellular location of various biochemical processes.
1 Ιαν 2011 · The fractionation of cells involves two distinct phases: disruption of the tissue or cells in a suitable medium and the subsequent separation of the subcellular particles, by differential centrifugation which exploits differences in their size and density.
