Αποτελέσματα Αναζήτησης
Populations of cells can be analyzed biochemically by disrupting them and fractionating their contents by ultracentrifugation. Further fractionations allow functional cell-free systems to be developed; such systems are required to determine the molecular details of complex cellular processes.
A cellular fractionation approach permits the quantitative evaluation of the subcellular localizations of small molecule compounds independent of their fluorescent properties [272]. Generally, following incubation with drugs, cells are separated into fractions, including the cytosol, nuclei, and cytoplasmic organelles.
30 Ιαν 1997 · Basic separation techniques needed in all cell biology laboratories are given and presented in the context of a number of related topics such as the labelling of ligands, the kinetics of their internalization and analysis of data, and the electron microscopy of organelles.
Here, we present a broad overview of recent approaches that take a comprehensive and “-omics” view to identify novel components critical for cell division, to understand the function of the cell division machinery, and to analyze the pathways and other novel factors that contribute to cell division.
In cell biology, cell fractionation is the process used to separate cellular components while preserving individual functions of each component. [1] This is a method that was originally used to demonstrate the cellular location of various biochemical processes.
Cell fractionation is a laboratory technique used to separate cellular components based on their size, density, and shape. This method allows scientists to isolate specific organelles or parts of a cell for further analysis, making it essential in understanding cellular functions and structure.
1 Ιαν 2011 · The fractionation of cells involves two distinct phases: disruption of the tissue or cells in a suitable medium and the subsequent separation of the subcellular particles, by differential centrifugation which exploits differences in their size and density.
