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The colony forming unit (CFU) is a measure of viable colonogenic cell numbers in CFU/mL. These are an indication of the number of cells that remain viable enough to proliferate and form small colonies. Isolated hMSCs were plated in a 6-well cell culture plate along with 2–3 mL of DMEM medium.
A simple method for the enumeration of bacteria and fungi is based on the quantification of colony-forming units (CFU) per ml or g of sample. For this, we must prepare serial dilutions of the sample, plate the diluted suspensions and count the number of colony forming units. Figure 1 shows a scheme of the method. Figure 1. CFU count method scheme.
The only way to understand dilution theory well is to practice it, so you should work practice problems until you feel confident in using dilution factors and calculating CFU/mL in original samples. You should also be able to determine the proper dilutions to use to obtain 30-300 colonies on a plate if the original number of CFU/mL in a sample ...
Give at least three real-world examples why determining bacterial numbers is an important technique. Explain how the standard plate count approach works. Calculate CFU of an original sample. Explain how absorbance can be used as is a measure of sample turbidity and cell numbers.
We then quantify the colony forming units (CFU) per mL, the original quantity extracted from the original culture, by multiplying the final dilution factor by the number of colonies that appear on the plate after growth occurs. The goal is to count between 25 and 250 colonies to ensure accurate reading, so the plate closed to that range is used.
20 Σεπ 2008 · Abstract. Aims: To develop a flexible and fast colony forming unit quantification method that can be operated in a standard microbiology laboratory. Methods and Results: A miniaturized plating method is reported where droplets of bacterial cultures are spotted on agar plates.
13 Νοε 2012 · The most common method is bacterial plating, which has the advantage of allowing live cell assessment through colony forming unit (CFU) counts but is not well suited for high throughput screening (HTS).
