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The colony forming unit (CFU) is a measure of viable colonogenic cell numbers in CFU/mL. These are an indication of the number of cells that remain viable enough to proliferate and form small colonies.
20 Σεπ 2008 · Abstract. Aims: To develop a flexible and fast colony forming unit quantification method that can be operated in a standard microbiology laboratory. Methods and Results: A miniaturized plating method is reported where droplets of bacterial cultures are spotted on agar plates.
The only way to understand dilution theory well is to practice it, so you should work practice problems until you feel confident in using dilution factors and calculating CFU/mL in original samples.
20 Μαρ 2012 · To assess accuracy and performance of the colony counter, we tested automated colony counting of different agar plates with known CFU numbers of S. pneumoniae, P. aeruginosa and M. catarrhalis...
13 Νοε 2012 · The widely used gold standard method is Colonies Forming Units (CFU) counting on plates . The CFU method has two noteworthy advantages, namely the capacity for counts of any number of bacteria using dilutions, if too many, or concentrations if too few.
Give at least three real-world examples why determining bacterial numbers is an important technique. Explain how the standard plate count approach works. Calculate CFU of an original sample. Explain how absorbance can be used as is a measure of sample turbidity and cell numbers.
In microbiology, a colony-forming unit (CFU, cfu or Cfu) is a unit which estimates the number of microbial cells (bacteria, fungi, viruses etc.) in a sample that are viable, able to multiply via binary fission under the controlled conditions.
