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In microbiology, a colony-forming unit (CFU, cfu or Cfu) is a unit which estimates the number of microbial cells (bacteria, fungi, viruses etc.) in a sample that are viable, able to multiply via binary fission under the controlled conditions.
25 Ιουλ 2020 · A “colony formation unit” per milliliter (CFU/mL) represents the number of living cells in one milliliter of sample. CFU/mL is used specifically for measuring biological contaminants like molds, fungus, and some bacteria.
Give at least three real-world examples why determining bacterial numbers is an important technique. Explain how the standard plate count approach works. Calculate CFU of an original sample. Explain how absorbance can be used as is a measure of sample turbidity and cell numbers.
You should also be able to determine the proper dilutions to use to obtain 30-300 colonies on a plate if the original number of CFU/mL in a sample is known. Watch Video 1 on how to perform a serial dilution using "pour plates" and the associated calculations:
The CFU count is crucial for quantifying bacterial growth and contamination levels in microbiological studies. CFUs are typically expressed as CFU per milliliter (CFU/mL) for liquids or per gram (CFU/g) for solids. Not all microbes will form colonies; some may be viable but non-culturable (VBNC).
The colony forming unit (CFU) is a measure of viable colonogenic cell numbers in CFU/mL. These are an indication of the number of cells that remain viable enough to proliferate and form small colonies.
3 Απρ 2024 · In microbiology, CFU is used to determine the number of viable bacterial cells in a sample per mL. Hence, it is usually used to indicate the degree of contamination in samples of water, vegetables, soil or fruits, or the magnitude of the infection in humans and animals. And by “viable” it means it includes only living cells (not the dead cells).
