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30 Νοε 2017 · The CRISPR/Cas9 system is a powerful, revolutionary tool for genome editing. However, it is not without limitations. There are PAM-free and CRISPR-tolerant regions that cannot be modified by...
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25 Οκτ 2024 · CRISPR multi-gene editing technology greatly enhances the scope and efficiency of gene editing and transcriptional regulation, particularly suitable for intervening in the functions of multiple ...
28 Ιουλ 2015 · CRISPR Handbook – Enabling Genome Editing and Transforming Life Science Research. Free PDF download on the CRISPR/Cas9 genome editing revolution, including CRISPR KO & KI workflows, case studies, and references.
10 Ιουν 2024 · To enable epigenome editing, we developed the first nuclease-deactivated dCas9 in living cells, to programmably target and control gene expression, without altering the DNA sequence.
15 Οκτ 2024 · CellEDIT, described in a recent webinar by Dr. Tobias Beyer, Chief Scientific Officer and Application Scientist at Cytosurge, addresses these issues by offering a novel approach. By using a FluidFM® Nanosyringe, the smallest needle in the world, to inject CRISPR/Cas9 ribonucleoproteic (RNP) complexes directly into the nucleus of single cells ...
15 Μαΐ 2018 · CRISPR-Cas9 is no longer just a gene-editing tool; the application areas of catalytically impaired inactive Cas9, including gene regulation, epigenetic editing, chromatin engineering, and...
15 Μαΐ 2018 · Here, we will present a brief history of gene-editing tools and describe the wide range of CRISPR-based genome-targeting tools. We will conclude with future directions and the broader impact of CRISPR technologies.