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10 Αυγ 2022 · Coagulase test is used to differentiate Staphylococcus aureus (positive) which produce the enzyme coagulase, from S. epidermis and S. saprophyticus (negative) which do not produce coagulase. i.e Coagulase Negative Staphylococcus (CONS).
For a negative tube coagulase test from catalase-positive, Gram-positive cocci in clusters that have creamy white colonies, report as “coagulase-negative staphylococci.” A positive slide coagulase test can be reported as S. aureus; however, the test should be confirmed with a tube test from nonhemolytic or only slightly hemolytic colonies ...
18 Ιαν 2022 · Lower B- Tube coagulase test for free coagulase. The tube on the left is positive, exhibiting a clot. The tube on the right is negative. Above: Negative coagulase test in Staphylococcus epidermidis (left) and positive coagulase tests in Staphylococcus aureus (center and right).
The coagulase test differentiates strains of Staphylococcus aureus from S. epidermidis and other coagulase-negative species. S. aureus strains are usually capable of coagulating EDTA-treated plasma in the tube test and will produce clumps of cells in the slide test.
The coagulase-positive staphylococci constitute the most pathogenic species S aureus. The coagulase-negative staphylococci (CNS) are now known to comprise over 30 other species. The CNS are common commensals of skin, although some species can cause infections.
If the slide coagulase test reaction is negative, inoculate a tube of rabbit plasma overnight and check for a clot in the tube. This is considered a positive reaction for free coagulase. Inoculate a tube containing ½ ml of rabbit plasma with the bacterial inoculum.
Results. Compared to the conventional microbiology testing the real‐time PCR assay had a higher detection rate for both S. aureus and coagulase‐negative Staphylococci (CoNS; 55 vs. 32 for S. aureus and 63 vs. 24 for CoNS). Hands‐on time preparing DNA, carrying out the PCR, and evaluating results was less than 5 h. Conclusions.
