Αποτελέσματα Αναζήτησης
31 Μαρ 2020 · A confocal microscope blocks the latter, resulting in a sharp image from the focal plane alone (Fig. 1a,b). With a high-resolution objective lens, a confocal microscope can generate optical...
24 Δεκ 2022 · Confocal microscopy is a non-invasive fluorescent imaging technique that uses lasers of various colors to scan across a specimen with the aid of scanning mirrors. The point of illumination is brought to focus in the specimen by the objective lens.
Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [ 1 ]
The available fluorescent probes include dyes for specific components, such as nucleic acids, reporters for particular ions such as calcium, modified DNA and RNA sequences, which are used for fluorescent in situ hybridization, and antibodies raised against almost any biological component imaginable. Fluorescent detection has the advantage of ...
Confocal microscopy, developed and patented by Marvin Minsky in 1955, uses optical imag-ing to create a virtual slice or plane, many micrometers deep, within the tissue. It provides very-high-quality images with fine detail and more contrast than conventional microscopy.
Confocal microscopy provides a means of rejecting the out-of-focus light from the detector such that it does not contribute blur to the images being collected. This technique allows for high-resolution imaging in thick tissues.
30 Νοε 2022 · Why the confocal microscope is used to obtain blur-free images by optical sectioning. How the confocal microscope works. When to select the confocal microscope to image fluorescent specimens. How to operate a single beam-scanning confocal microscope. How to improve signal-to-noise ratios (SNR) in your images.
