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1 Οκτ 2007 · The confocal microscope, based on the principle of confocal imaging, is a recently introduced and invaluable tool for high-resolution fluorescence microscopy. View Show abstract
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The confocal microscope, based on the principle of confocal...
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Confocal microscopy is a powerful tool that creates sharp images of a specimen that would otherwise appear blurred when viewed under a conventional microscope. This is achieved
Confocal microscopes work by exciting the fluorescent labels present in a specimen. The excitation is provided by lasers, as against the use of mercury and xenon lamps in standard epifluorescence technique.
THEORY Acousto-Op0c Tunable Filter. The wavelength of the diffracted light is dependent on the acoustic frequency in the quartz. By tuning the frequency of the acoustic wave, the desired wavelength of the optical wave can be diffracted acousto-optically.
INTRODUCTION. Confocal microscopy is a powerful tool for visualizing fluorescent specimens. The principal advantage of confocal microscopy over conventional wide-field microscopy is that it can reveal the three-dimensional struc-ture of the specimen.
Confocal microscopy improves the optical resolution and contrast of your images by reducing out of focus light. Talk outline... Basics of fluorescence microscopy and the principle of confocal microscopy. Multi-dimensional image acquisition with a point scanning confocal microscope. Advanced applications. Jablonski diagram Stokes shift.
Confocal microscopy, developed and patented by Marvin Minsky in 1955, uses optical imag-ing to create a virtual slice or plane, many micrometers deep, within the tissue. It provides very-high-quality images with fine detail and more contrast than conventional microscopy.
