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19 Ιαν 2023 · In microscopy, the term “resolution” is used to describe the ability of a microscope to distinguish details of a specimen or sample. In other words, the minimum distance between 2 distinct points of a specimen where they can still be seen by the observer or microscope camera as separate entities.
24 Δεκ 2022 · Magnification is the ability to make small objects seem larger, such as making a microscopic organism visible. Resolution is the ability to distinguish two objects from each other. Light microscopy has limits to both its resolution and its magnification.
4 Μαΐ 2021 · This presentation summarizes key concepts about microscopes and microscopy. It defines important terminology like magnification, resolution, resolving power, and numerical aperture. It explains that magnification increases apparent size but resolution is the ability to distinguish between two points.
14 Ιουν 2020 · Magnification, Resolving power, Principles and Applications of Simple, Compound, Stereozoom, Phase contrast, Fluorescent and Electron microscopes (TEM & SEM). Microscopy is the technical field that uses microscopes to observe samples which are not in the resolution range of the normal-unaided eye. Read more.
Different types of microscope have different resolving powers. Light microscopes let us distinguish objects as small as a bacterium. Electron microscopes have much higher resolving p ower – the most powerful allow us to distinguish individual atoms. Download a PDF of this infographic here.
This lecture discusses various criteria for resolution, the factors that influence resolution in the lateral and axial planes, and how to sample an image adequately using a camera or confocal microscope, such that the full optical resolution is retained.
22 Μαρ 2020 · The lateral resolution of an optical microscope can be estimated by imaging a standard microscope test specimen—a slide containing various patterns with different spatial frequencies or a biological specimen such as a diatom.