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Dermatophytosis is conventionally diagnosed using direct microscopic examination, gold standard culturing, and other identification techniques as microcultures and biochemical tests. This is a long, time-consuming process, requiring experts and specific diagnostic protocol [6].
9 Ιουν 2021 · In clinical laboratories, identification of dermatophytes classically relies on morphological traits of the fungus from macroscopic and microscopic examination of the cultures, thus requiring skill and expertise.
11 Φεβ 2020 · Dermatophytes are the aetiological factors of a majority of superficial fungal infections. What distinguishes them from other pathogenic filamentous fungi is their unique ability to degrade keratin.
Real-time PCR is a rapid and sensitive approach to detect an organism directly from clinical samples. As real-time PCR assay is conducted in a closed tube system, it limits the risk of contamination and helps in detection of multiple species of dermatophytes using different species-specific probes.
Dermatophytoses are mycoses caused by pathogenic fungi that generally trigger superficial infections in animals and particularly in humans. These dermatophytoses are mainly caused by filamentous fungi that can invade and feed on keratinized substrates [1] such as skin, hair, and nails [2].
The biological diagnosis of dermatophytosis allows: (i) to confirm the diagnosis of mycosis, first with direct microscopic examination of biological samples; (ii)to identify the fungus involved, especially for epidemiological purposes; and (iii)to follow up the therapeutic efficacy, particularly in the context of contagious Tinea capitis.
17 Δεκ 2020 · The conventional gold standard diagnostic method is a direct microscopic examination followed by 3 to 4 weeks of Sabouraud’s dextrose agar (SDA) culturing, and it may require further post-culturing identification through biochemical tests or microculture technique application.