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Understand how to quantify bacterial cells. Learn how to solve a dilution problem. It is a common practice to determine microbial counts for both liquid and solid specimens--suspensions of E. coli in nutrient broth all the way to soil samples and hamburger meat.
To make a 10 mM glucose from the 1 M (=1000 mM) glucose stock solution: 10 mM/1000 mM = 1/100 So, this is a 100-fold dilution or a 1e-2 ("ten to the minus two") dilution. You could take 1 ml of the 1 M glucose and add 99 ml of water to get 100 ml total solution.
The following problem sets test your ability to calculate dilution factors and concentration * s. Dilution Factor calculation. Concentration of a dilution calculation. Number of cells transferred calculation. Antibiotic concentration from stock solution calculation.
plate 1: 10^-2. plate 2: 10^-4. plate 3: 10^-6. CFU/ml in the original sample (don't forget units!): 7.5 x 10^7 CFU/ml. Calculate the following. An example for how to type exponents into Canvas: 10^-1 (use the carrot: shift-6) Individual dilution factors at each tube.
Serial Dilution Practice Problem Set. The following problem sets test your ability to calculate dilution factors and concentration * s. Dilution Factor calculation. Concentration of a dilution calculation. Number of cells transferred calculation.
The following protocol is a step-by-step procedure to working dilution problems, and includes some practice problems at the end. The purpose can be determination of bacterial, fungal, or viral counts (commonly called colony - forming units, CFUs , for bacterial or fungal counts, or plaque - forming units, PFUs , for viral counts).
Practice dilution problems for the Bio 201 practical. 1. You do a dilution by combining 100 ml volume of MgCl plus 700 ml unit volumes of RO water. What is the dilution factor, i.e, how many more times dilute is it than the original concentration?
