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A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the order of the four bases: G (guanine), C (cytosine), A (adenine) and T (thymine). This is then reported as a text string, called a read.
Provides high resolution to obtain a base-by-base view of a gene, exome, or genome. Delivers quantitative measurements based on signal intensity. Detects virtually all types of genomic DNA alterations, including single nucleotide variants, insertions and deletions, copy number changes, and chromosomal aberrations.
DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine.
In its commercial version, 454 sequencing can read up to 20 million bases per run by applying the pyrosequencing technique on picotiter plates that facilitate sequencing of large amounts of...
Next-generation sequencing (NGS) is a massively parallel sequencing technology that offers ultra-high throughput, scalability, and speed. The technology is used to determine the order of nucleotides in entire genomes or targeted regions of DNA or RNA.
Whole-genome sequencing (WGS) and whole-exome sequencing (WES) provide the sequence of DNA bases across the genome and exome, respectively. Whole-transcriptome sequencing provides sequence information about coding and multiple noncoding forms of RNA to assess variations and gene expression levels across the entire transcriptome.
11 Οκτ 2017 · This review commemorates the 40th anniversary of DNA sequencing, a period in which we have already witnessed multiple technological revolutions and a growth in scale from a few kilobases to the...
