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Base Pairing – DNA and Transcription For the audio version of the file, click this link: Base Pairing Worksheet Audio The Central Dogma: DNA RNA Protein
After completing this, you would like to take a closer look at the sequence of nucleotides in the DNA fragment. You use a method called dideoxy Sanger sequencing. In this worksheet you will mimic the Sanger sequencing method to see how DNA is sequenced.
Worksheet: Mutations Practice There are three ways that DNA can be altered when a mutation (change in DNA sequence) occurs. 1. Substitution – one base-pairs is replaced by another: Example: G to C or A to G C G T C 2. Insertion – one or more base pairs is added to a sequence: Example: CGATGG –– CGAATGG GCTACC GCTTACC 3.
DNA Base Pairing Worksheet. There are base pairing rules for writing complimentary DNA strands for a given strand. Write the complimentary DNA strand for each given strand of DNA. Now write the mRNA strand for the given DNA strand. 7.
Sequencing DNA. Goal – obtain the string of bases that make a given DNA strand. Problem –Typically one cans sequence directly only DNA of short length (400-700 bp – Sanger; <200 - Illumina). Sequence assembly – the process of putting together the fragments.
Construct the right side of your DNA molecule by putting together in sequence a cytosine, thymine, guanine and adenine nucleotide. Complete the left side of the DNA ladder by adding complementary nucleotides or nucleotides that fit. Your finished model should resemble a ladder.
Sequencing provides students with an opportunity to use web-based resources and in-class activities to understand modern methods of DNA sequencing. Students will work together to determine the sequence of a hypothetical segment of DNA. Students can also make a bookmark that illustrates two methods of visualizing DNA sequence.
