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  1. 19 Φεβ 2011 · The method should allow prompt and accurate identification of bacteria. The principle of the method is simple; when a pure PCR product of the 16S gene is obtained, sequenced, and aligned against bacterial DNA data base, then the bacterium can be identified. Confirmation of identity may follow.

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      The Universal Method (UM) described here will allow the...

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      The potential products QUGP-Fn6. Rn2, 1096 bp and...

  2. 18 Μαρ 2019 · Antimicrobial susceptibility testing (AST) is the traditional method for assaying antimicrobial resistance in bacteria (Box 1). These culture-based tests determine how well bacteria can grow...

  3. 23 Σεπ 2016 · The use of PCR (polymerase chain reaction) to amplify the DNA present in a sample has transformed gene-based assays and overcomes the major challenge of rapid testing of samples in a POC healthcare setting, with the potential for the question ‘is it bacterial or not?’ to be answered quickly.

  4. With advances in DNA technology—notably PCR, DNA sequencing, and comparative sequence analysis—it has become faster and more accurate to identify microbes by comparing DNA sequences from small samples of isolated cells with comprehensive databases of bacterial and fungal DNA sequences. As a laboratory providing microbial testing

  5. 26 Αυγ 2016 · The acid/HPLC method is a new tool to determine DNA extraction efficiencies and should aid in the development of improved protocols for releasing DNA from a broad range of microorganisms. DNA-based testing is becoming the preferred method both for identifying microorganisms and for characterizing microbial communities.

  6. Clinical and Laboratory Standards Institute guideline MM18—Interpretive Criteria for Identification of Bacteria and Fungi by Targeted DNA Sequencing includes information intended for use with molecular diagnostic testing procedures published in CLSI documents MM031 and MM09.2 These guidelines contain information about developing, evaluating, and...

  7. 15 Απρ 2007 · A schematic for 16S rRNA (an ∼1500-base pair gene), located on the small ribosomal subunit (30S). Circles represent conserved regions that serve as gene targets for PCR amplification and DNA sequencing of bacteria.

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