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Gel electrophoresis is a method of separating DNA fragments by movement through a Jello-like substance called agarose. Derived from a seaweed polysaccharide, agarose gels form small pores
In this lab, we saw how gel electrophoresis could be used to determine the molecular composition and the specific charge. We were able to use gel electrophoresis successfully to determine how many different DNA, RNA, or proteins are present in a sample and how large they are in comparison to one another.
Agarose gel electrophoresis is method for separation (by size), quantifying, purification of nucleic acids fragments mixture, and analysis of DNA restriction fragments. It is one of the most widely-used techniques in biochemistry and molecular biology.
Learning Objectives: Upon completion of this activity, students will: Determine the presence and size of Wolbachia 16S rDNA amplified by PCR. Develop important skills in gel electrophoresis, a widely used technique for the preparation and analysis of DNA and proteins.
Objectives: To have students review agarose gel electrophoresis as a tool for separating DNA fragments. To allow students to investigate some of the factors affecting electrophoresis of DNA including: voltage, % agarose, fragment size, staining resolution, and linear DNA vs. circular DNA fragments.
21 Ιουν 2017 · Lesson 08 – Gelin’: Gel Electrophoresis Simulation: Lab Activity. Student will learn how DNA is separated into a fingerprint by using a chromatography activity to simulate gel electrophoresis. Download Lesson.
This lab practical describes the process of agarose gel electrophoresis to separate and analyze extracted DNA samples by size. Agarose gel electrophoresis involves placing DNA samples in wells in an agarose gel submerged in an electrophoresis buffer solution.
