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Eosin Methylene Blue (EMB) agar is a selective and differential culture medium. It selectively promotes the growth of Gram-negative bacteria and aids in the differentiation of lactose fermenter and non-lactose fermenting colonies. EMB Agar is used for the isolation of fecal coliforms.
4 Ιαν 2022 · EMB Agar (Eosin Methylene Blue Agar) is recommended for the isolation and differentiation of gram negative enteric bacteria from clinical and nonclinical specimens. It is useful in differentiating gram positive and gram-negative bacteria.
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EMB agar. Only gram-negative bacteria grow on EMB agar. (Gram-positive bacteria are inhibited by the dyes eosin and methylene blue added to the agar.) Based on its rate of lactose fermentation, E. coli produces dark, blue-black colonies with a metallic green sheen on EMB agar. Download Presentation. agar.
Purpose. Eosin-methylene blue agar is selective for gram-negative bacteria against gram-positive bacteria. In addition, EMB agar is useful in isolation and differentiation of the various gram-negative bacilli and enteric bacilli, generally known as coliforms and fecal coliforms respectively (8).
Eosin Methylene Blue agar (EMB agar) is both selective and differential culture medium ideally used for isolating faecal coliforms. It is a selective medium due to inhibition of gram-positive bacteria by methylene blue.
